Eytan Wine (PhD, University of Toronto, MD Tel-Aviv University)

Associate Professor

Department of Pediatrics

University of Alberta

Division of Pediatric Gastroenterology

ECHA Room 4-577, 11405 87 Ave.
Edmonton, Alberta

T6G 1C9  Canada

Phone: 780-248-5420; Fax: 1-888-353-1157



Research Description

Host-microbial Interactions in Pediatric Inflammatory Bowel Diseases

Unravelling complex chronic diseases remains a critical gap in biomedical research. Intestinal microbes are involved in many systemic disorders, including diabetes, obesity, and cancer. The role of microbes in the pathogenesis of inflammatory bowel diseases (IBD, including Crohn disease and ulcerative colitis) is the main focus of the Wine lab. The laboratory investigates mechanisms of microbial virulence related to IBD using in vitro and in vivo disease models. Effects on host cell permeability and cytokine secretion, as well as factors that determine pathogenicity of resident microbes are actively investigated and supported by operating grants. These interests fit very well with the clinical expertise of Dr. Wine as a pediatric gastroenterologist, treating children with IBD. As a physician-scientist, Dr. Wine enjoys the opportunity to combine clinical and scientific goals and cover basic, clinical, and translational areas of research from bench-to-bedside in an academic center of excellence.

Work in the lab spans from in-vitro, cell-based experiments (focused on the inflammasome), through assessment of the intestinal barrier in children with IBD (using probe-based confocal endomicroscopy during endoscopy), to analysis of microbial samples collected at the site of host-microbe interaction. The lab has extensive experience in running patient-based microbial analysis, together with support from the Centre of Excellence for Gastrointestinal Inflammation and Immunity Research (CEGIIR) research team, which includes bioinformatics experts and computer analysts. Another focus (both clinical and research) is on the role of nutrition in gut homeostasis and disease. Together, the basic/clinical/translational work in the Wine lab is aimed to better understand the pathogenesis of IBD, especially in children, in order to identify novel therapeutic options.

Selected Publications

  • M Bording-Jorgensen, M Alipour, G Danesh, E Wine. ATP-induced inflammasome activation enhances Citrobacter rodentium clearance mediated by ROS.Cellular Physiology and Biochemistry 2017;41:193-204.  Abstract
  • D Zaidi, L Churchill, HQ Huynh, MW Carroll, E Wine. Quantitative analysis of capillary flow rates in the duodenum of pediatric inflammatory bowel diseases patients. Journal of Pediatric Gastroenterology and Nutrition, Abstract
  • W Elhenawy, M Bording-Jorgensen, E Valguarnera, MF Haurat, E Wine, MF Feldman. LPS Remodelling triggers formation of Outer Membrane Vesicles in Salmonella. mBio 2016;7:e00940. Abstract
  • D Zaidi, M Bording-Jorgensen, JF Turcotte, JJ Liu, C Sergi, HQ Huynh, M Carroll, E Wine. Increased intestinal epithelial cell shedding in the duodenum of children with inflammatory bowel diseases. Accepted to Journal of Pediatric Gastroenterology and Nutrition 2016; 644-50. Abstract
  • M Alipour, D Zaidi,  R Valcheva, J Jovel, I Martinez, C Sergi, J Walter, GK Wong, LA Dieleman, MW Carroll, HQ Huynh, E Wine. Mucosal barrier disruption and loss of bacterial diversity are primary abnormalities in paediatric ulcerative colitis. Journal of Crohn’s and Colitis, 2016; 10:462-71. Abstract
  • G Zhang, D Sharon, J Jovel, L Liu, E Wine, N Tahbaz, S Indik, A Mason. Pericentriolar targeting of the Mouse mammary tumor virus GAG protein. PLoS One 2015;10:e0131515. Abstract
  • W Wang, J Jovel, B Halloran, E Wine, J Patterson, G Ford, S O’Keefe, B Meng, D Song, Y Zhang, Z Tian, ST Wasilenko, M Rahbari, S Reza, T Mitchell, T Jordan, E Carpenter,  K Madsen, R Fedorak, L Dielemann, GK Wong, AL Mason. Metagenomic analysis of microbiome in colon tissue from subjects with inflammatory bowel diseases reveals interplay of viruses and bacteria. Inflammatory Bowel Diseases 2015;21:1419-27. Abstract
  • R Li, K Navab, G Hough, N Daher, M Zhang, D Mittelstein, K Lee, P Pakbin, A Saffari, M Bhetraratana, D Sulaiman, T Beebe, L Wu, N Jen, E Wine, J Araujo, A Fogelman, C Sioutas, M Navab, TK Hsiai. Exposure to Atmospheric Ultrafine Particles Promotes Intestinal Free Oxidative Fatty Acid Production. Environmental Health Perspectives 2015;123:34-41. Abstract
  • HM Becker, D Grigat, S Ghosh, GG Kaplan, L Dieleman, E Wine, RN Fedorak, R Panaccione, HW Barkema. Living with Inflammatory Bowel Disease: A Crohn’s and Colitis Canada Survey. Canadian Journal of Gastroenterology 2015;29:77-84. Abstract
  • D Zaidi, E Wine. An update on travelers’ diarrhea. Current Opinion in Gastroenterology 2015;31:7-13. Abstract
  • E Wine. Host-microbe interactions in the gut: lessons learned from models of inflammatory bowel diseases. LymphoSign Journal 2014;1:61-76.
  • SY Salim, J Jovel, E Wine, GG Kaplan, R Vincent, A Thiesen, HW Barkema, KL Madsen. Early Exposure to Ingested Airborne Pollutant Particulate Matter Increases Mucosal Exposure to Bacteria and Induces Early Onset of Inflammation in Neonatal IL-10 Deficient Mice. Inflammatory Bowel Diseases 2014;20:1129-38. Abstract
  • M Alipour, Y Lou, M Bording-Jorgensen, D Zimmerman, C Sergi, JJ Liu, E Wine. A Balanced IL-1β Activity is Required for Host Response to Citrobacter rodentium Infection. PLoS One 2013;8:e80656. Also featured in: World Biomedical Frontiers: http://biomedfrontiers.org/infection-2014-5-27. Abstract
  • E Wine, DR Mack, J Hyams, AR Otley, J Markowitz, WV Crandall, N Leleiko, AM Muise, AM Griffiths, D Turner. Interleukin-6 is associated with steroid resistance and reflects disease activity in pediatric severe ulcerative colitis. Journal of Crohn’s and Colitis 2013;7:916-22.  Abstract
  • A Levine, E Wine. Effects of exclusive enteral nutrition on Crohn's disease: Clues to the impact of diet on disease pathogenesis. Inflammatory Bowel Diseases 2013;19:1322-9.M. Abstract
  • Gordon, M El-Kalla, Z Yeuwen, Y Fiteih, J Law, N Volodko, A Mohamed, AOS El-Kadi, L Liu, J Odenbach, A Thiesen, C Onyskiw, H Abu Ghazaleh, J Park, S Bong Lee, VC Yu, C Fernandez-Patron, RT Alexander, E Wine, S Baksh. The tumour suppressor gene, RASSF1A, is essential for protection from inflammation-induced injury. PLoS One 2013;8:e75483.  Abstract
  • JC Ossa, NK Ho, E Wine, N Yeung, SD Gray-Owen, PM Sherman. Adherent-invasive Escherichia coli blocks interferon-gamma-induced signal transducer and activation of transcription (stat)-1 in human intestinal epithelial cells. Cellular Microbiology 2013;15:446-57. Abstract
  • JJ Liu, EM Davis, E Wine, Y Lou, JK Rudzinski, P Boulanger, AL Thiesen, C Sergi, RN Fedorak, KL Madsen, R Irvin. Epithelial cell extrusion leads to breaches in the intestinal epithelium. Inflammatory Bowel Diseases 2013;19:912-21. Abstract
  • L Kish, N Hotte, GG Kaplan, R Vincent, R Tso, M Ganzle, KP Rioux, A Thiesen, HW Barkema, E Wine, KL Madsen. Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. PLoS One 2013;8:e62220.  Abstract
  • S Michail, M Durbin, D Turner, AM Griffiths, D Mack, J Hyams, N Leleiko, H Kenche, A Stolfi, E Wine. Alterations in the gut microbiome in children with severe ulcerative colitis. Inflammatory Bowel Diseases 2012;18:1799-808. Abstract
  • JF Turcotte, K Wong, SJ Mah, LA Dieleman, D Kao, K Kroeker, B Claggett, JR Saltzman, E Wine, RN Fedorak, JJ Liu. Increased epithelial gaps in the small intestine are predictive of hospitalization and surgery in patients with inflammatory bowel disease. Clinical and Translational Gastroenterology 2012;3:e19. Abstract
  • LA Beamish, AR Osornio-Vargas, E Wine. Air pollution: an environmental factor contributing to intestinal disease. Journal of Crohn’s and Colitis 2011;5:279-86. (Featured on Journal Cover). Abstract
  • MG Gareau, E Wine, DM Rodrigues, JH Cho, MT Whary, G MacQueen, DJ Philpott, PM Sherman. Bacterial Infection Causes Stress-Induced Memory Dysfunction in Mice. Gut 2011;60:307-17; Abstract Accompanying Commentary: Gut 2011; 60:288-9.
  • E Wine, G Shen-Tu, MG Gareau, HA Goldberg, C Licht, BY Ngan, ES Sorensen, J Greenaway, J Sodek, R Zohar, PM Sherman. Osteopontin mediates colonic epithelial cell hyperplasia and attaching-effacing lesions in response to Citrobacter rodentium infection. The American Journal of Pathology 2010;177:1320-32. Abstract
  • E Wine, JC Ossa, SD Gray-Owen, PM Sherman. Adherent-invasive Escherichia coli target the epithelial barrier. Gut Microbes 2010;1:80-4. Abstract
  • MG Gareau, E Wine, C Reardon, PM Sherman. Probiotics prevent death caused by Citrobacter rodentium infection in neonatal mice. Journal of Infectious Diseases 2010;201:81-91. Abstract
  • E Wine, MG Gareau, K Johnson-Henry, PM Sherman. Strain-specific probiotic (Lactobacillus helveticus) inhibition of Campylobacter jejuni invasion of human intestinal epithelial cells. FEMS Microbiology Letters 2009;300:146-52. Abstract
  • E Wine, JC Ossa, SD Gray-Owen, PM Sherman. Adherent-invasive Escherichia coli, strain LF82 disrupts apical junctional complexes in polarized epithelia. BMC Microbiology 2009;9:180. Abstract
  • E Wine, VL Chan, PM Sherman. Campylobacter jejuni mediated disruption of polarized epithelial monolayers is cell-type specific, time dependent, and correlates with bacterial invasion. Pediatric Research 2008;64:599-604. Abstract
  • E Wine, I Yaniv, IJ Cohen. Hyperimmunoglobulinemia in pediatric-onset type 1 Gaucher disease and effects of enzyme replacement therapy. Journal of Pediatric Hematology and Oncology 2007;29:451-7. Abstract
  • AM Muise, T Walters, E Wine, AM Griffiths, D Turner, RH Duerr, MD Regueiro, BY Ngan, W Xu, PM Sherman, MS Silverberg, D Rotin.  Equal contribution. Protein-tyrosine phosphatase sigma is associated with ulcerative colitis. Current Biology 2007;17:1212-8. Abstract
  • E Wine, SS Reif, E Leshinsky-Silver, B Weiss, RR Shaoul, R Shamir, D Wasserman, A Lerner, M Boaz, A Levine. Pediatric Crohn’s disease and growth retardation: the role of genotype, phenotype, and disease severity. Pediatrics 2004;114:1281-6. Abstract